Thursday, February 28, 2013

Low Level Entero Spiking

Low Level Entro Spiking Using Biomérieux Bioball
Principle:
In rehearse Enterobacteriaceae counts seen at side are often of a very menial level. Proficiency testing schemes challenge that gamey level contamination can be identified, but this does not reflect historical topics seen at site level. This method challenges that low level contamination can be identified. The Bioball contains a cognize number of colony forming units (30) and can therefore be employ for this purpose.
Method:
1.Aseptic techniques should be followed throughout preparation and testing.
2. put in one 30 cfu Bioball into a sterile container with chapeau.
3.Add 1 ml of Maximum Recovery Diluent to the container, allow the Bioball to totally dissolve.
4.Dispense 9 ml of product ( draw/cream) into the container, return the lid and leave the dissolving agent for approximately 10 minutes.
5.Vortex the solution to secure through mixing.
6.Label Petri dish with sufficient instruction to enable a full identification of the spiked examine and using a 1 ml pipette/dispenser enthral 1 ml of spiked sample directly into the Petri dish.
7. bourgeon the photographic plate with VRBGA and incubate for 2 ± 2h
8.Negative control plate of the original product sample should be made to ensure any counts achieved are as a direct result of the sample spiking.

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Results:
10 ml spiked sample contains 30 cfu-1 ml should contain approximately 3 cfu. The acceptable manoeuver range for results is 1-5 cfu. Results should be recorded, with any out of target results describe to the Technical Team Leader/Technical Manager.

Milk-sample used for milk was TESCO STD 2Pt UB 03 FEB @ 10:40
test DETAILSPLATE COUNT / CFU
banish CONTROL1 ML MILK0

SPIKED SAMPLE1ML SPIKED SAMPLE (BIOBALL/1ML electronic dictionary/9ML MILK)2
1ML SPIKED SAMPLE (BIOBALL/1ML machine readable dictionary/9ML MILK)2
1ML SPIKED SAMPLE (BIOBALL/1ML machine readable dictionary/9ML MILK)1
1ML SPIKED SAMPLE (BIOBALL/1ML MRD/9ML MILK)1
1ML SPIKED SAMPLE (BIOBALL/1ML MRD/9ML MILK)1
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